Blog
Guides, tutorials, and best practices for qPCR data analysis.
March 13, 2026
SYBR Green vs TaqMan: When to Use Each Chemistry
A practical guide to choosing between SYBR Green and TaqMan for your qPCR experiments — based on cost, specificity, and what your project actually needs.
March 13, 2026What Happened to qbase+ — and Why I Built VoilaPCR
I went looking for qbase+ and it was gone. So I built a browser-based qPCR analysis tool that does what qbase+ did, without the pain.
March 6, 2026Choosing the Best Reference Genes for qPCR
How to pick, validate, and use the right housekeeping genes for accurate normalization in RT-qPCR experiments.
March 6, 2026How to Calculate DDCt (Livak Method): A Complete Guide
Learn the delta-delta Ct method step by step, including the math, assumptions, common mistakes, and worked examples.
March 6, 2026qPCR Troubleshooting Guide: 10 Common Problems and Fixes
A practical checklist for diagnosing and fixing the most common qPCR issues, from NTC amplification to unexpected fold changes.