Upload your qPCR file, get publication-ready analysis in seconds. No installs, no spreadsheets, no headaches.
Used by researchers at
Independent researchers across four continents use VoilaPCR for their qPCR analysis.
From raw file to clean results in three steps.
Drop your .xlsx, .xls, .csv, or native instrument file. We auto-detect the format.
Pick your reference gene, control group, and analysis method. Or let the defaults run.
Get fold-change tables, QC reports, and publication-ready figures. Export or share instantly.
No sign-up required. Your data never leaves your browser.
Upload your file and see results in seconds.
Drop your spreadsheet here
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.xlsx, .xls, .csv, .rdml, or QuantStudio .eds — QuantStudio, CFX, LightCycler, ABI 7500, Biomark, and more
Everything you need for rigorous, reproducible qPCR analysis.
Eight diagnostic checks run on every analysis: NTC contamination, replicate scatter, reference-gene stability, late Ct, undetermined wells, extreme fold-change, amplification efficiency, and identical-Ct flags. Each finding names the well and the number behind it.
Journal presets for Nature, Cell, PLOS ONE, and Science. Palettes, bar colors, fonts, axis labels, error bars, significance brackets, dot overlays, and figure dimensions in inches. Download as SVG or 300-DPI PNG, or export straight to Prism.
Drop in one plate or several. Biological replicates run on separate plates are merged into one experiment, sample names are grouped automatically, and instrument formats can be mixed freely.
Click a screenshot to enlarge. Screens shown with example data: three plates, four groups.
Auto-detected on upload. No manual format selection needed.
QuantStudio 3/5/6/7
Thermo Fisher
StepOne / StepOnePlus
Applied Biosystems
ABI 7500 / 7500 Fast
Applied Biosystems
CFX96 / CFX384 / Opus
Bio-Rad
LightCycler 96 / 480
Roche
Rotor-Gene Q / 6000
QIAGEN
Stratagene Mx3000P/3005P
Agilent
Mastercycler realplex
Eppendorf
Mic qPCR (48-well)
Bio Molecular Systems
BioMark HD / EP1
Fluidigm
Generic CSV
Any instrument
All parsing, QC, and analysis run entirely in JavaScript on your machine. No file is uploaded to any server. Your raw data is never transmitted, stored, or accessible to anyone but you.
Our QC checks are aligned with the MIQE guidelines (Bustin et al., 2009). Each diagnostic references the relevant MIQE criterion so reviewers can verify compliance.
Use this citation in your methods section: