Upload your qPCR file, get publication-ready analysis in seconds. No installs, no spreadsheets, no headaches.
From raw file to clean results in three steps.
Drop your .xlsx, .xls, .csv, or native instrument file. We auto-detect the format.
Pick your reference gene, control group, and analysis method. Or let the defaults run.
Get fold-change tables, QC reports, and publication-ready figures. Export or share instantly.
Upload your file and see results in seconds.
Drop your spreadsheet here
or click to browse
.xlsx, .xls, or .csv — QuantStudio, CFX, LightCycler, ABI 7500, Biomark, and more
Everything you need for rigorous, reproducible qPCR analysis.
Eight diagnostic checks run instantly: NTC contamination, replicate scatter, reference-gene stability, late Ct, undetermined wells, extreme fold-change, amplification efficiency, and identical-Ct flags.
Export bar charts styled for Nature, Cell, or PNAS with one click. Customize colors, fonts, error bars, and significance markers, then download as SVG or PNG.
Analyze one plate or combine biological replicates across multiple plates in a single run. Mix instrument formats freely — VoilaPCR merges and normalizes automatically.
Auto-detected on upload. No manual format selection needed.
QuantStudio 3/5/6/7
Thermo Fisher
StepOne / StepOnePlus
Applied Biosystems
ABI 7500 / 7500 Fast
Applied Biosystems
CFX96 / CFX384 / Opus
Bio-Rad
LightCycler 96 / 480
Roche
Rotor-Gene Q / 6000
QIAGEN
Stratagene Mx3000P/3005P
Agilent
Mastercycler realplex
Eppendorf
Mic qPCR (48-well)
Bio Molecular Systems
BioMark HD / EP1
Fluidigm
Generic CSV
Any instrument
Coming Soon
VoilaPCR is the first tool in the VoilaScience suite. Coming soon: VoilaAssay — ELISA and plate reader analysis.
All parsing, QC, and analysis run entirely in JavaScript on your machine. No file is uploaded to any server. Your raw data is never transmitted, stored, or accessible to anyone but you.
Our QC checks are aligned with the MIQE guidelines (Bustin et al., 2009). Each diagnostic references the relevant MIQE criterion so reviewers can verify compliance.
Use this citation in your methods section: